Journal: European Journal of Immunology

Article Title: Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy

doi: 10.1002/1521-4141(200211)32:11%3C3235::AID-IMMU3235%3E3.0.CO;2-J

Figure Lengend Snippet: Fig. 3. Peritumoral CpG ODN treatment further increases the therapeutic effect of a contralateral DC- and CpG ODN-based vac- cine resulting in CD8 T cell-dependent complete regression of large established tumors. Mice with established tumors (mean size of 89 mm2) remained untreated (A, individual mice; n=10), or were treated with injections of CpG ODN into the margin of the tumor (B, peritumoral; n=5), with mature tumor antigen-pulsed DC coinjected with CpG ODN into the contralateral flank (C; n=9), or with a combination of peritumoral injection of CpG ODN with contralateral coinjection of CpG ODN and antigen-loaded DC (D; n=7). A total of four injections was performed in weekly intervals, starting on day 14 (indicated by arrows). (E) Mean tumor sizes of treatment groups were monitored and are plotted until the first mouse in each group reached a tumor size of 400 mm2. (F) Sur- vival of mice (tumor size of 400 mm2) without treatment (n=10), with peritumoral injection of CpG ODN combined with contralat- eral coinjection of CpG ODN and antigen-loaded DC (n=7), and with the same treatment but with depletion of CD8 T cells (n=5).

Article Snippet: Bone marrow leukocytes from mouse tibia and femur were depleted of T and B lymphocytes, and granulocytes by incubation with rat anti-mouse CD4, CD8, Ly6G, CD45R and MHC II mAb (PharMingen, San Diego, CA and Serotec, Oxford, GB) followed by incubation (15 min, 4°C) with goat anti-rat IgG conjugated to magnetic beads (Dynal, Oslo, Sweden) and separation in a Dynal MPC-1 magnetic field (Dynal).

Techniques: Injection